Immunohistochemical Analysis of Mouse Brain

Brains from representative mice in each group (N = 2–3 per group) were post-fixed overnight followed by paraffin embedding. 5 μm thick sections from cortex were immuno-stained with MAP2 (catalog# AB5622; Millipore, Darmstadt, Germany; 1:500), GFAP (catalog# ab7260; Abcam, 1:5000), and Gli1 (sc-20687; Santacruz, 1:100) and processed as described previously^{13 (link)}. ImageJ software was used to quantify IHC data in Fig. 3 and 4 as described previously^{13 (link)}.

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Singh V.B., Singh M.V., Piekna-Przybylska D., Gorantla S., Poluektova L.Y, & Maggirwar S.B. (2017). Sonic Hedgehog mimetic prevents leukocyte infiltration into the CNS during acute HIV infection. Scientific Reports, 7, 9578.

Publication 2017

GFAP sc-20687

Brains Cortex Gfap Map2 Mice

Corresponding Organization : University of Rochester Medical Center

Other organizations : University of Nebraska Medical Center

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Variable analysis

independent variables

Mouse group

dependent variables

MAP2 expression
GFAP expression
Gli1 expression

control variables

Brain tissue processing (post-fixing, paraffin embedding)
Cortex section thickness (5 μm)
Immunostaining protocol (antibodies, concentrations)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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