Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were determined using a clinical chemistry analyzer Idexx VetTest 8008 (Idexx Laboratories, Westbrook, ME, USA) as described28 . Liver triglycerides were extracted with a 2:1 chloroform:methanol mixture and measured using the EnzyChrom Triglyceride Assay Kit (BioAssays Systems, Hayward, CA)29 (link).
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