Characterizing ASMP-Nano Formulations by DLS

The average hydrodynamic diameter (D_h) and polydispersity index (PDI) of all ASMP-Nano formulations were determined by measuring the rate of fluctuations in laser light intensity scattered by the nanomicelles immediately after their preparations using the Dynamic Light Scattering (DLS) analysis (Beckman Coulter® N4 Plus, Beckman Coulter s.r.l., Milan, Italy). Five minutes prior to light-scattering measurements each sample was diluted with ultrapure water previously filtered through 0.45 μm pore size filter (polyethersulfone membrane, Millipore Express PLUS, Merck, Italy). The final concentration was chosen on the basis of the measurement intensity ranging from 5 × 10⁴ to 1 × 10⁶ counts per second (cps). The average size for each ASMP-Nano formulation was obtained on three different samples for which six runs were carried out using an angle of 90° [30 (link)] and run time of 200 s at 20 °C.
The same protocol analysis was performed to characterise the Nano1HA_BFITC-CyA formulation.