Male C57BL/6 mice were maintained for 6 months on a high calorie diet (HCD) with high fat (35.8%) and high carbohydrate (36.8%), consisting of a standard pelleted feed, which was mixed with butter and sugar. Pork fat was given in addition to the feed to accelerate the development of obesity and impaired glucose tolerance. After the development of impaired glucose and insulin tolerance (after 6 months), animals were randomised by weight and divided into 4 groups (n = 7–13): (1) “Intact control”: vehicle (distilled water + 2 drops of Tween 80) and standard pelleted feed; (2) “Negative control”: vehicle (distilled water + 2 drops of Tween 80) + HCD; (3) “Positive control”: metformin at a dose of 250 mg/kg + HCD; (4) “Composition”: composition of leuzea and cranberry meal extracts at a dose of 70:500 mg/kg + HCD. All compounds were given once a day by oral gavage for 4 weeks.
Body weight was recorded weekly, and an oral glucose tolerance test was performed after 4 weeks. At the end of experiment, mice were decapitated, blood was taken for biochemical examination, the liver and pancreas for histology.
Body weight was recorded weekly, and an oral glucose tolerance test was performed after 4 weeks. At the end of experiment, mice were decapitated, blood was taken for biochemical examination, the liver and pancreas for histology.
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