Trichrome stainings were performed in 5-μm paraffin midhilar renal cross-sections to assess fibrosis by a computer-aided image analysis program (AxioVision 4.8.2, Carl Zeiss Microscopy, Thornwood, NY). In each slide, trichrome staining was semiautomatically quantified in 6-10 fields, expressed as fraction of kidney surface area, and the results from all fields averaged. Glomerular score (% of sclerotic out of 100 glomeruli) was assessed as described.(24 (link)) CD31 (1:50; AbD Serotec, UK) immunofluorescence was used to investigate capillary density.(31 (link)) Microvascular remodeling was assessed by wall-to-lumen ratio using α-smooth muscle actin (α-SMA) staining (1:50; Dako, Glostrup, Denmark).(32 (link)) Tubular injury was scored as described previously.(33 (link)) Oxidative stress indicated by in-situ production of superoxide anion was quantified in 30 μm DHE stained slides.(34 (link))
Protocol detail
Renal Histopathological Analysis
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Actin
Capillary
Fibrosis
Glomeruli
Immunofluorescence
Injury
Kidney
Microscopy
Oxidative stress
Paraffin
Sclerotic
Smooth muscle
Superoxide anion
Corresponding Organization : Mayo Clinic
Other organizations : Xuzhou Medical College, Affiliated Hospital of Xuzhou Medical College
Top 5 similar protocols
Variable analysis
independent variables
- Trichrome stainings
- CD31 immunofluorescence
- α-smooth muscle actin (α-SMA) staining
- Tubular injury scoring
- In-situ production of superoxide anion quantification
- Fibrosis (as fraction of kidney surface area)
- Glomerular score (% of sclerotic out of 100 glomeruli)
- Capillary density
- Microvascular remodeling (wall-to-lumen ratio)
- Tubular injury score
- Oxidative stress (superoxide anion production)
- 5-μm paraffin midhilar renal cross-sections
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!