The protein levels of iNOS and TRPV4 were detected by Western blotting.
20 (link) The primary antibodies were as follows: rabbit anti‐iNOS (1:1000, Cell Signaling Technology, USA), rabbit anti‐TRPV4 (1:500, Abcam, USA), and mouse anti‐β‐actin (1:5000, Sigma‐Alrich).
20 (link) The primary antibodies were as follows: rabbit anti‐iNOS (1:1000, Cell Signaling Technology, USA), rabbit anti‐TRPV4 (1:500, Abcam, USA), and mouse anti‐β‐actin (1:5000, Sigma‐Alrich).
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