The N and P concentrations of the dry sample were measured using the micro-Kjeldahl method and the molybdenum blue method, respectively [92 (link),93 (link)].
According to Wang et al., the PNUE was given by: [94 (link)]
Anmax, the maximum net photosynthetic rate; Narea, total foliar N.
N allocation within the photosynthetic apparatus was calculated according to the model from Niinemets et al. [95 (link)].
PNcb, the fraction of nitrogen allocated to the carboxylation system; Vcmax, maximum ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) carboxylation rate; 6.25, 6.25 g Rubisco (g nitrogen in Rubisco)−1 converts nitrogen content to protein content; Vcr, the maximum rate of Ribulose-1,5-bisphosphate (RUBP) carboxylation per unit Rubisco protein (20.8 μmol CO2 (g Rubisco)−1 s−1); Narea, total foliar N.
PNet, the fraction of nitrogen allocated to electron transport components; Jmax, maximum electron transport rate; 8.06, the investment in bioenergetics is at least 8.06 μmol cyt f (g N in bioenergetics)−1; Jmc, the potential rate of photosynthetic electron transport per unit cytochrome f (155.6 μmol electrons (μmol cyt f)−1 s−1); Narea, total foliar N.
PNlc, the fraction of nitrogen allocated to the light capture system; Cc, Total chlorophyll concentration; Narea, total foliar N; CB, chlorophyll-binding (2.15 mmol (g N)−1).
PNnon-psn is the fraction of nitrogen allocated to non-photosynthetic nitrogen.
According to Wang et al., the PNUE was given by: [94 (link)]
Anmax, the maximum net photosynthetic rate; Narea, total foliar N.
N allocation within the photosynthetic apparatus was calculated according to the model from Niinemets et al. [95 (link)].
PNcb, the fraction of nitrogen allocated to the carboxylation system; Vcmax, maximum ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) carboxylation rate; 6.25, 6.25 g Rubisco (g nitrogen in Rubisco)−1 converts nitrogen content to protein content; Vcr, the maximum rate of Ribulose-1,5-bisphosphate (RUBP) carboxylation per unit Rubisco protein (20.8 μmol CO2 (g Rubisco)−1 s−1); Narea, total foliar N.
PNet, the fraction of nitrogen allocated to electron transport components; Jmax, maximum electron transport rate; 8.06, the investment in bioenergetics is at least 8.06 μmol cyt f (g N in bioenergetics)−1; Jmc, the potential rate of photosynthetic electron transport per unit cytochrome f (155.6 μmol electrons (μmol cyt f)−1 s−1); Narea, total foliar N.
PNlc, the fraction of nitrogen allocated to the light capture system; Cc, Total chlorophyll concentration; Narea, total foliar N; CB, chlorophyll-binding (2.15 mmol (g N)−1).
PNnon-psn is the fraction of nitrogen allocated to non-photosynthetic nitrogen.
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