Total RNA was extracted from 4A+, hmox1, and ho1su1 using the TransZol plant kit as recommended by the manufacturer (Transgen, Beijing, China). Contaminating genomic DNA was removed using a DNaseI treatment. RNA was reverse transcribed using an oligo dT primer (Zhang et al., 2018 (link)) and the M-MLV reverse transcriptase (Takara, Kusatsu, Shiga, Japan). cDNA obtained from the reverse transcription reaction was used as a template for the amplification of the coding sequence of HMOX1 using the primers detailed in Supplementary Table 1 .
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