Cell Line Authentication and Characterization

LNCaP, PC-3, DU145, 22Rv1, and HEK-293T cells were obtained from the American Type Culture Collection, and cultured as described (33 (link)). All the cell lines were authenticated on April 1, 2015, by the method of short tandem repeat profiling at the Genetica DNA Laboratories. Enzalutamide was purchased from Selleck Chemicals (Houston, TX). The following antibodies were used in Western blot analyses: anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Millipore), anti-AR (N-20, Santa Cruz), anti-AR-V7 (Precision Antibody), and anti-FLAG (M2, Sigma).

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Zhan Y., Zhang G., Wang X., Qi Y., Bai S., Li D., Ma T., Sartor O., Flemington E.K., Zhang H., Lee P, & Dong Y. (2016). Interplay Between Cytoplasmic and Nuclear Androgen Receptor Splice Variants Mediate Castration Resistance. Molecular cancer research : MCR, 15(1), 59-68.

Publication 2016

LNCaP DU145 HEK-293T Enzalutamide anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, anti-AR (N-20, anti-FLAG (M2,

293t cells Antibodies Antibody Cell lines Enzalutamide Gapdh Glyceraldehyde 3 phosphate dehydrogenase Short tandem repeat Western blot analyses

Corresponding Organization :

Other organizations : Tulane University, Jilin University, New York University

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Variable analysis

independent variables

Enzalutamide

dependent variables

Protein expression levels of GAPDH, AR, AR-V7, and FLAG

control variables

LNCaP, PC-3, DU145, 22Rv1, and HEK-293T cell lines
Cell culture conditions (as described in reference 33)

controls

Positive control: None specified
Negative control: None specified

Annotations

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