Adipocyte Diameter Distribution Analysis

Diameter distributions in each adipocyte preparation were determined as reported by digital analysis of suspended cells using a Nikon Eclipse 80i microscope and Nikon Digital DXM 1200C camera. Digital images were analyzed using Nikon NIS-Elements (NE) Br software, generating mean diameters (with distribution), in micrometers (μ), for each preparation. The mean cell surface area and cell volume, in μ² and pl, respectively, were computed from the diameters ^{20 (link), 21 (link)}.

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Ge F., Walewski J.L., Torghabeh M.H., Lobdell H I.V., Hu C., Zhou S., Dakin G., Pomp A., Bessler M., Schrope B., Ude-Welcome A., Inabnet W.B., Feng T., Carras-Terzian E., Anglade D., Ebel F.E, & Berk P.D. (2015). Facilitated Long Chain Fatty Acid Uptake by Adipocytes Remains Upregulated Relative to BMI for More Than a Year After Major Bariatric Surgical Weight Loss. Obesity (Silver Spring, Md.), 24(1), 113-122.

Publication 2015

Eclipse 80i microscope Digital DXM 1200C camera NIS-Elements (NE)

Adipocyte Cell Microscope

Corresponding Organization : Columbia University Irving Medical Center

Other organizations : Cornell University, Mount Sinai Beth Israel

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Variable analysis

independent variables

Diameter distributions in each adipocyte preparation

dependent variables

Mean diameters (with distribution), in micrometers (μ)
Mean cell surface area, in μ^2
Mean cell volume, in pl

control variables

Suspended cells using a Nikon Eclipse 80i microscope and Nikon Digital DXM 1200C camera
Digital images analyzed using Nikon NIS-Elements (NE) Br software

controls

No positive or negative controls were explicitly mentioned in the input.

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