The following procedures were previously described. For single-cell sequencing, white blood cells were isolated with the Erythroclear Red Blood Cell Depletion Reagent Kit (STEMCELL Technologies, catalog no. 01738), according to the manufacturer’s protocol. Cells were washed with PBS with 0.02% bovine serum albumin. To obtain single-cell gel beads in emulsion (GEMs), we resuspended cells at a concentration of 1000 cells/μl and loaded the mix on the Chromium Comptroller Instrument (10x Genomics). Single-cell cDNAs and libraries were prepared with Chromium Single-Cell 3′ Library and Gel Bead Kit v3.1 (10x Genomics, catalog no. 1000121). Briefly, GEM-RT incubation was performed in a C1000 Touch thermal cycler with 96-deep well reaction module (Bio-Rad, catalog no. 1851197). Single-strand cDNAs were purified with DynaBeads MyOne Silane Beads (Thermo Fisher Scientific, catalog no. 37002D) and amplified with the C1000 cycler. Amplified cDNA products were cleaned with 0.6X DynaBeads MyOne Silane Beads (Thermo Fisher Scientific, catalog no. 37002D). Quality and quantity of the cDNAs were assessed on 4200 TapeStation (Agilent Technologies) with High Sensitivity D5000 DNA ScreenTape (Agilent, catalog no. 5067-5592). Libraries were diluted to the same molarity and pooled for sequencing on NovaSeq6000 (Illumina) sequencers (23 (link)).