DHA27 Synthesis and Cytokine Analysis

DHA27 was synthesized by College of Pharmacy, Zunyi Medical University and College of Pharmacy, Army Medical University (the Third Military Medical University) (Song et al., 2016 (link)). Gentamicin, tobramycin (TOB), and amikacin were purchased from Beijing Solarbio Science & Technology Co., Ltd. whereas daunorubicin (DNR) was purchased from Med Chem Express (USA). RNA iso Plus and Reverse Transcription Kit were purchased from TaKaRa (Japan). Primer design and synthesis were performed by Sangon Biotech (Shanghai) Co., Ltd. Interleukin (IL)-1β (mouse) and interferon (IFN)-γ (mouse) ELISA kits were purchased from Thermo Fisher Scientific (USA).

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Wang N., Chen X., Luo J., Deng F., Shi F., Wu Q., Huang Y., Ouyang Q., Qin R, & Zhou H. (2022). Artemisinin derivative DHA27 enhances the antibacterial effect of aminoglycosides against Pseudomonas aeruginosa by inhibiting mRNA expression of aminoglycoside-modifying enzymes. Frontiers in Pharmacology, 13, 970400.

Publication 2022

Gentamicin tobramycin amikacin daunorubicin RNA iso Plus Reverse Transcription Kit

Amikacin Daunorubicin Elisa Gentamicin Ifn γ Interferon Interleukin 1β Military Mouse Primer Reverse transcription Synthesis Tobramycin

Corresponding Organization : Army Medical University

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Variable analysis

independent variables

DHA27 (synthesized by College of Pharmacy, Zunyi Medical University and College of Pharmacy, Army Medical University)
Gentamicin (purchased from Beijing Solarbio Science & Technology Co., Ltd.)
Tobramycin (TOB) (purchased from Beijing Solarbio Science & Technology Co., Ltd.)
Amikacin (purchased from Beijing Solarbio Science & Technology Co., Ltd.)
Daunorubicin (DNR) (purchased from Med Chem Express (USA))

dependent variables

Measured outcomes not explicitly mentioned

control variables

RNA iso Plus and Reverse Transcription Kit (purchased from TaKaRa (Japan))
Primer design and synthesis (performed by Sangon Biotech (Shanghai) Co., Ltd.)
Interleukin (IL)-1β (mouse) and interferon (IFN)-γ (mouse) ELISA kits (purchased from Thermo Fisher Scientific (USA))

controls

Positive control not explicitly mentioned
Negative control not explicitly mentioned

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