In a separate set of mice (n = 5), the kidneys of the mice were extracted following sacrifice and immediately processed to evaluate the DNA damage as per the standard Comet assay protocol [29 (link),30 (link)]. The DNA damage was assessed by measuring the length of DNA migration, which included the diameter of the nucleus and the migrated DNA, using image analysis Axiovision 3.1 software by Carl Zeiss (White Plains, NY, USA).
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