HEK293 Cell Culture and Transfection
Corresponding Organization : University of Edinburgh
Variable analysis
- Transfection of HEK293 cells with channel cDNAs and acyl thioesterases or empty pcDNA3.1 plasmid in controls
- Not explicitly mentioned
- HEK293 cells were originally obtained from ATCC and cultured and transfected essentially as previously described
- HEK293 cells were maintained in DMEM containing 10% fetal bovine serum, incubated at 37 °C in 5% CO2, and used between passage 18 and 30
- HEK293 cells used in this study do not express endogenous BK channel subunits as determined by mRNA, protein, or functional assays
- Positive control: Transfection of channel cDNAs
- Negative control: Transfection of empty pcDNA3.1 plasmid
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