Mice were anesthetized with Avertin and euthanized by exsanguination after severing the left axillary artery. BAL fluid (BALF) was harvested by lavaging the lungs 3x using PBS (Gibco). BALF was centrifuged for 10 min at 1000 g at 4°C to separate cells from BAL supernatant. Supernatants were stored at −80°C until cytokine evaluation. After collecting BALF, lungs were removed and homogenized using GentleMACS tubes (Miltenyi Biotech, Westphalia, Germany) in 1 mL DMEM (HyClone) and stored at −80°C as previously described.64 (link)
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Bronchoalveolar Lavage and Lung Tissue Collection
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Corresponding Organization : University of Georgia
Other organizations : Trellis Bioscience (United States)
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Variable analysis
independent variables
- Anesthesia with Avertin
- Cytokine levels in BAL supernatant
- Lung homogenate composition
- Exsanguination by severing left axillary artery
- BAL fluid (BALF) collection by lung lavage with PBS
- Centrifugation of BALF to separate cells and supernatant
- Storage of BALF supernatant and lung homogenate at -80°C
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