Yulink Knockdown in Zebrafish Embryos

Zebrafish embryos were obtained by natural mating, and MO microinjection was performed at the 1 ~ 4 cell stage. The YULINK-MO antisense oligonucleotide (5′-GGCAGGACAGTGGCTTGTTCAGTGC-3′) and its 5 bp mismatch MO negative control (5′-GGCtGcACAGTcGCTTcTTCAcTGC-3′) were used following our previous publication [13 (link)]. Embryos positioned in an agarose injection chamber were injected with 5–10 ng of MO in 4.6 nl of Danieu buffer (58 mM NaCl, 0.7 mM KCl, 0.4 mM MgSO₄, 0.6 mM Ca(NO₃)₂, 5 mM HEPES, pH 7.6) using a Narishige micromanipulator and needle holder (Narishige, Tokyo, Japan). The specificity of the YULINK-MO was demonstrated in our previous publication [13 (link)].