Sections from nodose ganglia of mice previously injected with viruses for monosynaptic retrograde rabies tracing were processed for in situ hybridization of Glp1r and Oxtr mRNA using a previously-optimised modification of the RNAscope assay 62 (link). Sections were cut at 10μm on a cryostat and collected on Superfrost Plus slides, then allowed air-dry at room temperature for one hour. Slides were then dipped in molecular grade ethanol and further air-dried overnight at room-temperature. RNAscope in situ hybridization was performed on these sections using the RNAscope Multiplex Fluorescent Kit v2 (Advanced Cell Diagnostics) as per the manufacturer’s instructions, with a modification to the pre-treatment procedure (Protease IV incubation conducted for 20 min at room temperature) that allows for preservation of the fluorescent reporter signal while also providing optimal signal from the target mRNAs. Probes for Glp1r, Oxtr and appropriate positive (Ubc) and negative (DapB) controls (detailed in Methods Table 1) were hybridized and after completion of the procedure slides were immediately cover slipped using Prolong Antifade medium.