Anti-EpCAM mAb, EpMab-37 was previously established (25 (link)). To generate recombinant EpMab-37, VH cDNA of EpMab-37 and CH of mouse IgG2a was cloned into the pCAG-Ble vector. VL cDNA of EpMab-37 and CL cDNA of mouse kappa light chain were also subcloned into the pCAG-Neo vector (FUJIFILM Wako Pure Chemical Corporation). The vector for the recombinant EpMab-37 was transduced into BINDS-09 (FUT8-knockout ExpiCHO-S) cells using the ExpiCHO Expression System (Thermo Fisher Scientific, Inc.), as previously described (29 (link)-36 (link)). EpMab-37-mG2a-f was purified using Ab-Capcher (ProteNova Co., Ltd.). Mouse IgG (cat. no. 140-09511) and IgG2a (cat. no. M7769) were purchased from FUJIFILM Wako Pure Chemical Corporation and MilliporeSigma, respectively. 281-mG2a-f [defucosylated anti-hamster podoplanin (PDPN) mAb, control mouse IgG2a for ADCC reporter bioassay] was previously described (37 ).
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Recombinant Production of Anti-EpCAM mAb EpMab-37
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Other organizations : Tohoku University, Microbial Chemistry Research Foundation
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Variable analysis
independent variables
- Generation of recombinant EpMab-37 antibody
- Purification of recombinant EpMab-37 antibody
- Mouse IgG (cat. no. 140-09511) and IgG2a (cat. no. M7769) were used as controls
- 281-mG2a-f [defucosylated anti-hamster podoplanin (PDPN) mAb, control mouse IgG2a for ADCC reporter bioassay] was used as a control
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