Total RNA was extracted and reversely transcribed using the PrimeScript RT reagent Kit (TaKaRa, Japan) according to the protocols recommended by the manufacturer. The cDNA was subjected to qRT-PCR detection using a SYBR Green Premix Kit (TaKaRa, Japan) [8 (link)]. The relative expression was calculated using the 2-ΔΔCT method for the following genes: VIM, CDH2, MME, MMP14, IL6, CD68, CD80, CD86, and THBS1.
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