Fasting EDTA plasma samples stored at 80 °C were used to measure concentrations of hsCRP using nephelometry on a BN II using reagents from Siemens (Eschborn, Germany) and IL-18 by Elisa technology using the IL-18 test kit from MBL (Nagoya, Japan). More details are previously published (Herder et al, 2013 (link)). Eighty-six participants were excluded from this analysis due to missing values; however, the number of participants with severe anxiety remained the same. Both, CRP and IL-18 concentrations were approximately lognormal distributed and therefore log-transformed to be used as continuous variables.
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Biomarkers of Inflammation and Anxiety
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Variable analysis
independent variables
- None explicitly mentioned
- HsCRP concentrations
- IL-18 concentrations
- Fasting EDTA plasma samples
- Plasma samples stored at -80 °C
- None explicitly mentioned
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