Histology and Immunostaining of MPNST Tumors

Sections from Roc or vehicle-treated MPNST tumors were prepared and stained with hematoxylin and eosin (H&E) or immunostained for p-histone H3(Ser¹⁰) (pH3; ab32107, Abcam) or CC3 (#9664, Cell Signaling) as previously described (32 (link)).

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Chang L.S., Oblinger J.L., Burns S.S., Huang J., Anderson L.W., Hollingshead M.G., Shen R., Pan L., Agarwal G., Ren Y., Roberts R.D., O’Keefe B.R., Kinghorn A.D, & Collins J.M. (2019). Targeting protein translation by rocaglamide and didesmethylrocaglamide to treat MPNST and other sarcomas. Molecular cancer therapeutics, 19(3), 731-741.

Publication 2019

ab32107

Eosin Histone h3 Mpnst Tumors

Corresponding Organization : The Ohio State University

Other organizations : Center for Cancer Research, National Cancer Institute, Target (United States)

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Variable analysis

independent variables

Treatment with Roc or vehicle

dependent variables

Staining intensity for p-histone H3 (Ser10) (pH3)
Staining intensity for cleaved caspase-3 (CC3)

control variables

Sections from MPNST tumors

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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