Immunoblotting Analysis of SKA1 and SKA3

Cells were harvested 48 h after transfection, and lysates were prepared. Immunoblotting was performed with anti-SKA1 antibodies (1:500 dilution, SAB2701430; Sigma-Aldrich, St. Louis, MO, USA) and anti-SKA3 antibodies (1:1,000 dilution, ab175951; Abcam, Cambridge, UK). Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies (1:10,000 dilution, ab8245; Abcam) were used as an internal loading control. The procedures were described previously [29 (link), 42 (link), 43 (link)].

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Yamada Y., Arai T., Kojima S., Sugawara S., Kato M., Okato A., Yamazaki K., Naya Y., Ichikawa T, & Seki N. (2018). Anti-tumor roles of both strands of the miR-455 duplex: their targets SKA1 and SKA3 are involved in the pathogenesis of renal cell carcinoma. Oncotarget, 9(42), 26638-26658.

Publication 2018

SAB2701430 ab175951 ab8245

Anti antibodies Cells Dilution Glyceraldehyde 3 phosphate dehydrogenase Transfection

Corresponding Organization : Chiba University

Other organizations : Teikyo University Chiba Medical Center

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Variable analysis

independent variables

Transfection

dependent variables

Expression of SKA1 protein
Expression of SKA3 protein

control variables

Time of cell harvesting (48 h after transfection)
GAPDH as internal loading control

controls

Positive control: Not specified
Negative control: Not specified

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