Western Blot Analysis of Cellular Proteins

Western blot analysis was performed as standard procedures previously described [41 (link)]. The following primary antibodies directed against the antigens were used: CDH1 (1:1000, mouse monoclonal, Cell Signaling, Danvers, MA, USA), fibrillarin (1:2000, rabbit monoclonal, Cell Signaling), β-catenin (1:2000, rabbit monoclonal, Cell Signaling), Notch-1 (1:1000, rabbit polyclonal, Santa Cruz Biotechnology), p-Akt-T308 (1:1000, rabbit monoclonal, Cell Signaling), total-Akt (1:2000, mouse monoclonal, Cell Signaling), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:5000, Vazyme). GAPDH was used as the loading control in the Western blots.

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Song H., Zhang Y., Liu N., Zhao S., Kong Y, & Yuan L. (2016). miR-92a-3p Exerts Various Effects in Glioma and Glioma Stem-Like Cells Specifically Targeting CDH1/β-Catenin and Notch-1/Akt Signaling Pathways. International Journal of Molecular Sciences, 17(11), 1799.

Publication 2016

fibrillarin β-catenin Notch-1 p-Akt-T308 total-Akt GAPDH

Antibodies Antigens Cdh1 Fibrillarin Gapdh Glyceraldehyde 3 phosphate dehydrogenase Mouse Rabbit Western blots β catenin

Corresponding Organization : Southeast University

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Variable analysis

independent variables

Primary antibodies directed against the antigens: CDH1, fibrillarin, β-catenin, Notch-1, p-Akt-T308, total-Akt

dependent variables

Protein expression levels of CDH1, fibrillarin, β-catenin, Notch-1, p-Akt-T308, total-Akt

control variables

GAPDH (glyceraldehyde-3-phosphate dehydrogenase) used as the loading control in the Western blots

controls

GAPDH used as a positive control

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