Protocol detail

Microstructural Analysis of Femoral Bone

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The femurs were dissected after the peptides’ injection, and three-dimensional Micro CT analysis was performed using a Skyscan 1172 system (Bruker, Kontich, Belgium) at 55 kV (167 µA) and a 10 µm voxel size, as described.^{66 (link),67 (link)} The skeletal parameters were assessed by micro-CT and followed published nomenclature guidelines.^{68 (link)} Trabecular bone microarchitecture [trabecular bone volume fraction (BV/TV), trabecular bone thickness (Tb. Th), trabecular number (Tb. N), and trabecular separation (Tb. Sp)] was analyzed in a manually delineated region of interest 0.25–2.5 mm proximal to the distal femoral growth plate. Cortical thickness (Cs. Th) was 0.6 mm region at the femoral mid-diaphysis.

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Aljohani H., Stains J.P., Majumdar S., Srinivasan D., Senbanjo L, & Chellaiah M.A. (2021). Peptidomimetic inhibitor of L-plastin reduces osteoclastic bone resorption in aging female mice. Bone Research, 9, 22.

Publication 2021

Skyscan 1172 system

Cortical Diaphysis Femoral Growth plate Micro ct Peptides Skeletal Trabecular

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Variable analysis

independent variables

Peptides' injection

dependent variables

Trabecular bone volume fraction (BV/TV)
Trabecular bone thickness (Tb. Th)
Trabecular number (Tb. N)
Trabecular separation (Tb. Sp)
Cortical thickness (Cs. Th)

control variables

Femurs were dissected after the peptides' injection
Three-dimensional Micro CT analysis was performed using a Skyscan 1172 system at 55 kV (167 µA) and a 10 µm voxel size
Trabecular bone microarchitecture was analyzed in a manually delineated region of interest 0.25–2.5 mm proximal to the distal femoral growth plate
Cortical thickness (Cs. Th) was measured in a 0.6 mm region at the femoral mid-diaphysis

controls

No positive or negative controls were explicitly mentioned.

Annotations

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