Immunocytochemical Analysis of Cellular Markers

The procedures of immunocytochemistry were described previously^{19 (link)}. Nucleuses were stained with DAPI (1 μg/ml) or PI (2 μg/ml) containing RNase A (50 μg/ml). Samples were examined with a BZ-9000 inverted fluorescence microscope (Keyence). Anti-GFAP rabbit polyclonal antibodies (G4546, Sigma-Aldrich), anti-MBP rat monoclonal antibody (MAB386, Millipore), anti-Ki-67 rabbit polyclonal antibodies (AB9260, Millipore), anti-Hif1α rabbit polyclonal antibodies (NB100-479, Novus Biologicals), anti-Hif2α rabbit polyclonal antibodies (NB100-122, Novus Biologicals), Anti-CDKN2B (p15/INK4b) rabbit polyclonal antibodies (bs-4269R, Bioss Antibody), anti-RUNX1/AML1 rabbit polyclonal antibodies (ab23980, Abcam).

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Tokumoto Y., Tamaki S., Kabe Y., Takubo K, & Suematsu M. (2017). Quiescence of adult oligodendrocyte precursor cells requires thyroid hormone and hypoxia to activate Runx1. Scientific Reports, 7, 1019.

Publication 2017

BZ-9000 inverted fluorescence microscope MAB386 AB9260 NB100-479 NB100-122 ab23980

Aml1 Anti antibodies Anti gfap antibodies Antibodies Antibody Biologicals Dapi Fluorescence microscope Hif1α Immunocytochemistry Monoclonal antibody Novus Nucleuses Rabbit Rnase

Corresponding Organization : Keio University

Other organizations : National Center for Global Health and Medicine

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Variable analysis

independent variables

Anti-GFAP rabbit polyclonal antibodies (G4546, Sigma-Aldrich)
Anti-MBP rat monoclonal antibody (MAB386, Millipore)
Anti-Ki-67 rabbit polyclonal antibodies (AB9260, Millipore)
Anti-Hif1α rabbit polyclonal antibodies (NB100-479, Novus Biologicals)
Anti-Hif2α rabbit polyclonal antibodies (NB100-122, Novus Biologicals)
Anti-CDKN2B (p15/INK4b) rabbit polyclonal antibodies (bs-4269R, Bioss Antibody)
Anti-RUNX1/AML1 rabbit polyclonal antibodies (ab23980, Abcam)

dependent variables

Nucleuses stained with DAPI (1 μg/ml) or PI (2 μg/ml) containing RNase A (50 μg/ml)

control variables

Samples were examined with a BZ-9000 inverted fluorescence microscope (Keyence)

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