Quantifying Soybean SOD Gene Expression

Total RNA was extracted from soybean using the plant total RNA isolation kit (TIANGEN, Beijing, China), and the cDNAs were synthesized using the TransScript All-in-One First-Strand cDNA synthesis SuperMix for qPCR kit (TransGen Biotech, Beijing, China). qRT-PCR assays were performed using UtraSYBR Mixture (low ROX) and ABI 7500 sequencer. The GmGADPH was used as internal control (Huis, Hawkins & Neutelings, 2010 (link)). The SOD genes and GmGADPH primers are listed in supplementary Table S1. The three biological replicates were obtained and expression levels were calculated using 2^−ΔΔCt method and Student’s t-test (Livak & Schmittgen, 2001 (link)).

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Lu W., Duanmu H., Qiao Y., Jin X., Yu Y., Yu L, & Chen C. (2020). Genome-wide identification and characterization of the soybean SOD family during alkaline stress. PeerJ, 8, e8457.

Publication 2020

plant total RNA isolation kit TransScript All-in-One First-Strand cDNA synthesis SuperMix for qPCR kit ABI 7500 sequencer

Assays Biological Cdna Genes Isolation Plant rna Primers Soybean Student Synthesis

Corresponding Organization :

Other organizations : Harbin Normal University, Heilongjiang University, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression levels of SOD genes

control variables

GmGADPH gene (used as internal control)

controls

Positive control: None mentioned
Negative control: None mentioned

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