Fasting and Postprandial Lipid Profiles

We sampled venous blood before breakfast and after dinner, at baseline (Visit 1), after 5 days of HFD (Visit 2), and after a further 5 days on the HFD, either with daily exercise or no exercise (Visit 3) (Fig. 6). The participants fasted from 22:00 h the night before the blood sampling on all visits, with blood obtained between 07:15 and 07:45. The evening (postprandial) samples were obtained 34 (SD 7) minutes after eating dinner, between 19:20 and 20:00 h. The time since the last bout of exercise at Visit 3 was (by design) different between the EXam and EXpm group: For fasted samples this time was ⁓24 h for EXam and ⁓12 h for EXpm, and for postprandial samples the time was ⁓36 h for EXam and ⁓24 h for EXpm. Circulating concentrations of total cholesterol, HDL cholesterol, LDL cholesterol, and triglycerides were analysed in whole blood using Cobas b 101 (Roche Diagnostics Ltd, Switzerland), and have been reported previously^{15 (link)}.