Synthesis and Purification of Vitamin D Metabolites
Lumisterol, 7DHC, D3 and 1,25(OH)2D3 were obtained from Sigma-Aldrich (St. Louis, MO). 20(OH)7DHC, 20(OH)L3, pL, 17(OH)pL and 17,20(OH)2pL were synthesized as previously described32 (link), 34 (link), 43 (link); while 22(OH)L3, 20,22(OH)2L3 and 24(OH)L3 were produced from L3 enzymatically using purified bovine CYP11A140 (link). These compounds were purified by reverse-phase HPLC; their structures and purities were determined by NMR and mass spectrometry32 (link), 34 (link), 40 (link), 43 (link).
Slominski A.T., Kim T.K., Hobrath J.V., Janjetovic Z., Oak A.S., Postlethwaite A., Lin Z., Li W., Takeda Y., Jetten A.M, & Tuckey R.C. (2017). Characterization of a new pathway that activates lumisterol in vivo to biologically active hydroxylumisterols. Scientific Reports, 7, 11434.
Corresponding Organization : Birmingham VA Medical Center
Other organizations :
University of Dundee, Memphis VA Medical Center, University of Tennessee Health Science Center, National Institute of Environmental Health Sciences, National Institutes of Health, University of Western Australia
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