Sections approximately 6 µm thick were cut and collected on polylysine coated slides. Staining of section with 0.1% (w/v) toluidine blue in 1% (w/v) borax, pH 11 was done for general structure observations [35] (link).
To determine the location of starch deposits in cotyledons of ungerminated and germinated seeds, semithin sections of seeds were stained with I2-KI solution [25] (link). Slides were observed under light microscope (Nikon DS-Fi1).
To determine the location of starch deposits in cotyledons of ungerminated and germinated seeds, semithin sections of seeds were stained with I2-KI solution [25] (link). Slides were observed under light microscope (Nikon DS-Fi1).
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