Illumina and 454 Sequencing of Bacterial 16S rRNA

The obtained genomic DNA was then submitted to the Illumina high-throughput sequencing (samples S1 and S7) and 454 pyrosequencing (samples S1–S8), which were performed using the Illumina Hiseq 2000 and FLX Titanium platform of Roche 454 from the Beijing Genome Institute (Shenzhen, China), respectively. The bacterial 16S rRNA gene (V3-V4 region, approximately 460 bp) was carried out with Illumina-specific fusion primers V3F and V4R (Claesson et al., 2009 (link)). PCR amplification was conducted with previous report (Huang et al., 2014 (link)). The 12-bp barcode with primers was used to assign individual sequences to samples. The 16S rRNA gene amplicons were submitted to an Agilent 2100 Bioanalyzer (Agilnet, United States) before using FLX Titanium platform of 454 pyrosequencing (Roche, United States). The GenBank accession numbers for the genomic datasets in NCBI are SRX825942 and SRX825518.