Comprehensive Western Blot Analysis

Western blot was performed according to a standard protocol (Li et al., 2018 (link)). The primary antibodies used were the following: Cleaved-caspase3 (Abcam, Cambridge, MA, United States), Bcl-2 (Abcam, Cambridge, MA, United States), Bax (Abcam, Cambridge, MA, United States), RIPK1 (Proteintech, Wuhan, China), MLKL (Proteintech, Wuhan, China), SLC7A11 (Proteintech, Wuhan, China), GPX4 (Proteintech, Wuhan, China), LC3 (Proteintech, Wuhan, China), p62 (Proteintech, Wuhan, China), tubulin (Proteintech, Wuhan, China) and GAPDH (Proteintech, Wuhan, China) used a s the loading control.

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Zhu X., Wang S., Cheng Y., Gu H., Zhang X., Teng M., Zhang Y., Wang J., Hua W, & Lu X. (2023). Physiological ischemic training improves cardiac function through the attenuation of cardiomyocyte apoptosis and the activation of the vagus nerve in chronic heart failure. Frontiers in Neuroscience, 17, 1174455.

Publication 2023

Cleaved-caspase3 Bcl-2 Bax RIPK1 SLC7A11 tubulin GAPDH

Antibodies Bcl 2 Caspase3 Gapdh Gpx4 Ripk1 Tubulin Western blot

Corresponding Organization : Jiangsu Province Hospital

Other organizations : Nantong University, Affiliated Hospital of Nantong University, Sichuan University, West China Hospital of Sichuan University, Nanjing Maternity and Child Health Care Hospital

Top 5 similar protocols

Variable analysis

independent variables

Western blot protocol (Li et al., 2018)

dependent variables

Cleaved-caspase3
Bcl-2
RIPK1
SLC7A11

control variables

Tubulin
GAPDH

controls

Positive controls not explicitly mentioned.
Negative controls not explicitly mentioned.

Annotations

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