CH4 in the headspace of slurries was analyzed by gas chromatography as described previously [23 (link)]. Volatile fatty acids from centrifuged (10,000× g, 10 min) samples of the sediment slurries were analyzed by HPLC as described previously [23 (link)]. Data analyses were performed using ChromQuest (Thermo Scientific, Waltham, MA, USA) and Chromeleon software (Thermo Scientific, Waltham, MA). Sulfate and sulfide were quantified as described in [24 (link)].
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