BEAS-2B cells were treated with fucoxanthin and incubated with 10 ng/mL TNF-α/IL-4 for 24 h. Human monocytic THP-1 cells were purchased from the Bioresource Collection and Research Center (BCRC, Taiwan) and cultured in RPMI 1640 medium. The THP-1 cells were treated with calcein-AM solution (Sigma) for 30 min as described previously [26 (link)]. We co-cultured the THP-1 and BEAS-2B cells and detected the adherent THP-1 cells using fluorescence microscopy (Olympus, Tokyo, Japan).
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