Cadmium Quantification and Morphological Assessment in B. juncea

The cadmium standard curve (Abs = 0.46900C + 0.019858, R² = 0.997) was plotted using the Cd standard solution. B. juncea roots and leaves were prepared and digested according to the approach of Jiang et al. [40 (link)] and the Cd content was determined by Thermo Scientific iCE 3000 Series AA Spectrometers with three biological replicates. Thirty plants were randomly selected from each treatment group to measure the shoot height and root length with a ruler according to Ling et al. [89 (link)]. The dry weights of aboveground and underground parts were measured respectively according to Matse et al. [90 (link)]. Cut off the plant at the base of the stem with a blade and put it into an oven to dry to constant weight. Use an analytical balance to weigh the dry weight of the aboveground part and the underground part, respectively. The cell activity of root tip was determined by Evans blue staining according to Liu and Lin [91 ]. The root tip material was stained with 0.25% (w/v) Evans blue for 24 h, and photo-graphed under stereometric microscope. The data were analyzed by ANOVA using origin 9.1 software. The differences at p < 0.05 were shown to be significant by the LSD test.