Assessing RTX Effects on PAN-Induced Podocyte Defects

To examine the non-immunological effects of RTX on PAN induced cytoskeletal defects, podocytes were grown under growth restrictive conditions for 12 days and subsequently incubated with media containing 10% FBS in the presence of 30 µg/ml PAN (Sigma, Munich, Germany), 100 µg/ml MabThera (Roche, Basel, Switzerland) or the combination of both for 48 h. All experiments were performed at least three times starting on growth-restricted days 12–14 (methodology previously described in^{13 (link)}).

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Jeruschke S., Alex D., Hoyer P.F, & Weber S. (2022). Protective effects of rituximab on puromycin-induced apoptosis, loss of adhesion and cytoskeletal alterations in human podocytes. Scientific Reports, 12, 12297.

Publication 2022

MabThera

Cytoskeletal Growth conditions Mabthera Podocytes

Corresponding Organization :

Other organizations : Essen University Hospital

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Variable analysis

independent variables

30 µg/ml PAN (Sigma, Munich, Germany)
100 µg/ml MabThera (Roche, Basel, Switzerland)
The combination of both PAN and MabThera

dependent variables

Non-immunological effects of RTX on PAN induced cytoskeletal defects

control variables

Podocytes were grown under growth restrictive conditions for 12 days
Podocytes were subsequently incubated with media containing 10% FBS

controls

Positive control: Not specified
Negative control: Not specified

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