Pharmacological treatments in zebrafish

All pharmacological treatments were performed as described previously (López-Schier and Hudspeth, 2006 (link); Wibowo et al., 2011 (link); Pinto-Teixeira et al., 2015 (link)). Briefly, the following concentrations and timings used were: Neomycin sulfate (Sigma, St. Louis, MO) 250 µM for 45 min; N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine-t-butyl ester (DAPT) (Sigma) 100 µM for 24–48 hr. Equal amounts of DMSO were diluted in embryo medium for control specimens.

Free full text: Click here

Viader-Llargués O., Lupperger V., Pola-Morell L., Marr C, & López-Schier H. (2018). Live cell-lineage tracing and machine learning reveal patterns of organ regeneration. eLife, 7, e30823.

Publication 2018

Neomycin sulfate N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine-t-butyl ester (DAPT)

Dapt Dmso Embryo Ester Neomycin sulfate Pharmacological treatments

Corresponding Organization :

Other organizations : Helmholtz Zentrum München, Centre for Genomic Regulation, Senso (Spain)

Top 5 similar protocols

Variable analysis

independent variables

Neomycin sulfate (Sigma, St. Louis, MO) 250 µM for 45 min
N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine-t-butyl ester (DAPT) (Sigma) 100 µM for 24–48 hr

dependent variables

Not explicitly mentioned

control variables

Equal amounts of DMSO diluted in embryo medium for control specimens

controls

Positive control: Not specified
Negative control: Equal amounts of DMSO diluted in embryo medium for control specimens

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!