Quantitative Analysis of Cannabinoids and Terpenes

Acetonitrile, anhydrous ammonium formate, ethanol, formic acid, and hexanoic acid (>99%) were obtained from Sigma-Aldrich (HPLC grade, Saint Louis, MO, USA). Ultra-pure water was provided by the Milli-Q Plus system (Millipore Corp., Billerica, MA, USA). Cannabinoid analytical standards were purchased from RESTEK (RESTEK, Bellefonte, PA, USA): cannabidivarinic acid (CBDVA), cannabigerovarinic acid (CBGVA), cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabigerol (CBG), cannabidiol (CBD), (-)-Δ9-trans-tetrahydrocannabivarin (THCV), (-)-Δ9-trans-tetrahydrocannabivarinic acid (THCVA), cannabinol (CBN), cannabinolic acid (CBNA), cannabichromevarinic acid (CBCVA), (-)-Δ9-trans-tetrahydrocannabinol (Δ-9-THC), (-)-Δ8-trans-tetrahydrocannabinol (Δ-8-THC), cannabicyclol (CBL), cannabicyclolic acid (CBLA), cannabichromene (CBC), (-)-Δ9-trans-tetrahydrocannabinolic acid (THCA), and cannabichromenic acid (CBCA). Each of those standards was obtained at a stock concentration of 1000 µg/mL except CBLA which was obtained at a stock concentration of 500 µg/mL. Terpene standard mix at a stock concentration of 2500 µg/mL from each terpene containing the following terpenes—α-pinene, camphene, (-)-β-pinene, β-myrcene, δ-3-carene, α-terpinene, p-cymene, d-limonene, ocimene, γ-terpinene, terpinolene, linalool, (-)-isopulegol, geraniol, β-caryophyllene, α-humulene, nerolidol, (-)-guaiol, and (-)-α-bisabolol—was obtained from RESTEK (RESTEK, Bellefonte, PA, USA).