Immunofluorescence Microscopy of Ovarian Cancer Cells

The immunofluorescent microscopy was performed using methods previously described.⁷⁷ (link) Briefly, 1 × 10⁵ ovarian cancer cells (OVCAR3, OVCAR4 and PEO1) and negative control cells (OSEC) were seeded on coverslips and incubated overnight. The cells on coverslips were fixed in 4% PFA and incubated overnight with IgG antibodies extracted from ovarian tumour tissues diluted 1:100 in PBS and 1% FCS. The coverslips were then incubated with 1:200 anti-human IgG (Invitrogen, A-11013) and mounted with DAPI (Invitrogen, P36935). The images were taken using confocal microscope (Leica SP5). The confocal images were analysed using Fiji.