Histological Evaluation of Lung Tissue

Formaldehyde fixed lung tissue from male ND and HFD mice was collected from the control group as well as 1 h, 6 h, 24 h, 72 h and 192 h post-BTT and subsequently embedded in paraffin (Sakura, Germany). 5 μm thick tissue-sections were deparaffinized, rehydrated in decreasing alcohol series and subsequently stained in hematoxylin for three minutes. Afterwards the sections were blued using running water for 10 min and subsequently stained in eosin for 10 s [32 (link)]. The staining was completed by implementing dehydration of the slides in increasing alcohol series with incubation in Roti®-Histol. Sections were mounted with Entellan®. One area per animal out of a total of 6 animals per group was analyzed for the evaluation of the trauma. This number is based on the power analysis conducted for the animal experiment application (license number: 1183). Pictures were taken with the UC30 color camera at X10 and X40 magnification. All stainings were acquired with an Olympus IX81 microscope and analyzed with the Olympus software cellSens Dimensions 2.3 (Build 18,987).