Dihydroethidium (DHE) was acquired from Beyotime Biotech based in Shanghai, China. Primary renal tubular epithelial cells in the culture plate were incubated with 2 µM DHE in dark at 37 °C for 30 minutes. Then, wash the cells with new culture medium for three times and observe them under a fluorescence microscope.
Protocol detail
Quantifying Renal Oxidative Stress
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Corresponding Organization :
Other organizations : Huazhong University of Science and Technology, Tongji Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
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Variable analysis
independent variables
- Concentration of Dihydroethidium (DHE)
- Fluorescence intensity of primary renal tubular epithelial cells
- Time of incubation with DHE (30 minutes)
- Temperature of incubation (37 °C)
- Number of cell washes (3 times)
- No positive or negative controls were explicitly mentioned in the input.
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