Vero E6 cells (CRL-1586, American Type Culture Collection [ATCC]) and 293FT/hACE2+TMPRSS (18 (link)) were cultured in high glucose Dulbecco’s Modified Eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific) at 37°C with 5% CO2. Rat lung epithelial cells L2 (CCL-149, ATCC) were cultured in F-12K Medium (ATCC) supplemented with 10% FBS at 37°C with 5% CO2. Rat primary tracheal epithelial cells (Cell Biologics) were grown on culture flasks or plates pre-coated with gelatin-based coating solution (Cell Biologics) in Complete Epithelial Cell Medium (Cell Biologics) at 37°C with 5% CO2.
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Culturing Epithelial Cells for COVID-19 Research
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Corresponding Organization : Animal and Plant Health Inspection Service
Other organizations : Walter Reed Army Institute of Research, Rice University, Yale University
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independent variables
- Cell types: Vero E6 cells, 293FT/hACE2+TMPRSS cells, rat lung epithelial cells L2, and rat primary tracheal epithelial cells
- Not explicitly mentioned
- Culture conditions: 37°C with 5% CO2
- Culture media: High glucose DMEM (for Vero E6 and 293FT/hACE2+TMPRSS cells), F-12K Medium (for rat lung epithelial cells L2), and Complete Epithelial Cell Medium (for rat primary tracheal epithelial cells)
- Supplementation: 10% fetal bovine serum (FBS)
- Culture substrates: Culture flasks or plates pre-coated with gelatin-based coating solution (for rat primary tracheal epithelial cells)
- No positive or negative controls were explicitly mentioned in the provided information.
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