The CRC cell line HCT116 and normal human fibroblast cells (MRC-5) were obtained from the European Collection of Cell Cultures (Salisbury, UK).
HCT116 and MRC-5 cells were cultured as monolayers under standard conditions (37°C, 5% CO2) with whole-cell culture growth medium [10% fetal calf serum (FCS)] as previously described (40 (link)) and passaged when cells reached 70–80% confluency. A human T-lymphocyte cell line (Jurkat cells) was cultured in suspension with a whole-cell culture growth medium containing 10% FCS (41 (link)). Before all experiments, the cells were washed three times with serum-starved medium (3% FCS) and further incubated for 30 min with the same medium before initiation of experiments. All experiments were performed with the serum-starved medium.
HCT116 and MRC-5 cells were cultured as monolayers under standard conditions (37°C, 5% CO2) with whole-cell culture growth medium [10% fetal calf serum (FCS)] as previously described (40 (link)) and passaged when cells reached 70–80% confluency. A human T-lymphocyte cell line (Jurkat cells) was cultured in suspension with a whole-cell culture growth medium containing 10% FCS (41 (link)). Before all experiments, the cells were washed three times with serum-starved medium (3% FCS) and further incubated for 30 min with the same medium before initiation of experiments. All experiments were performed with the serum-starved medium.
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