Genetically Engineered Mouse Models for Breast Cancer

BRCA1^f/f and Tg (MMTV-Cre) 4Mam mice were obtained from the NCI Mouse Repository and JAX lab, respectively [46 (link), 47 (link)]. The generation of p16^−/−, p18^−/−, BRCA1^+/−, BRCA1^MGKO(BRCA1^f/f;MMTV-Cre or BRCA1^f/−;MMTV-Cre) mice was described previously [31 (link), 48 (link)–50 (link)]. The Institutional Animal Care and Use Committee at the University of Miami and Shenzhen University approved all animal procedures. Histopathology and immunohistochemistry (IHC) were performed as described previously [11 (link), 28 (link), 31 (link)]. The primary antibodies used were Ck14, cleaved caspase 3 (Thermal Scientific), PDGFRβ, phosphorylated PKCα (p-PKCα), phosphorylated FRA1 (p-FRA1), FRA1 (Cell signaling), ERα, BRCA1 (Santa Cruz), E-cadherin (E-cad), PKCα (BD Biosciences), and Vimentin (Vim) (Abcam). Immunocomplexes were detected by using the Vectastain ABC DAB kit according to the manufacturer’s instructions (Vector Laboratories) or by using FITC- or rhodamine-conjugated secondary antibodies (Jackson Immunoresearch).

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Bai F., Liu S., Liu X., Hollern D.P., Scott A., Wang C., Zhang L., Fan C., Fu L., Perou C.M., Zhu W.G, & Pei X.H. (2021). PDGFRβ is an essential therapeutic target for BRCA1-deficient mammary tumors. Breast Cancer Research : BCR, 23, 10.

Publication 2021

BRCA1 Vimentin (Vim) Vectastain ABC DAB kit FITC- or rhodamine-conjugated secondary antibodies

Animal Antibodies Brca1 Caspase 3 Ck14 E cadherin Fitc Immunohistochemistry Institutional animal care and use committee Mice Mmtv Pdgfrβ Pkcα Rhodamine Vector Vimentin

Corresponding Organization :

Other organizations : Shenzhen University Health Science Center, University of Miami, University of North Carolina at Chapel Hill

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Variable analysis

independent variables

Brca1^f/f
Tg (MMTV-Cre) 4Mam
p16^−/−
p18^−/−
Brca1^+/−
Brca1^MGKO (Brca1^f/f;MMTV-Cre or Brca1^f/−;MMTV-Cre)

dependent variables

Histopathology
Immunohistochemistry (IHC)

control variables

Institutional Animal Care and Use Committee at the University of Miami and Shenzhen University approved all animal procedures

controls

Positive controls not explicitly mentioned.
Negative controls not explicitly mentioned.

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