After a 72-h incubation of A. niger RAF106 in SDB with 2 μg/mL AFB1, the metabolites were extracted with chloroform as described above and separated using an Agilent 1260 series HPLC system equipped with an auto-injector and quaternary HPLC pump (Agilent Technologies, Santa Clara, CA, USA) [38 (link),46 (link),49 (link)]. The chromatography was conducted on a Phenomenex Luna 5u C18(2)100A column (250 × 4.60 mm, 5 μm) (Torrance, CA, USA). Samples (10 μL) were injected and eluted with the mobile solvent that contained 45:55 of methanol and water (v/v) with 0.1% formic acid. The total run time was 30 min with a flow rate of 0.5 mL/min. An MS analysis was performed with a Bruker maXis ESI Q-TOF (Bruker Daltonik, Bremen, Germany). The acquisition parameters were as follows: the capillary voltage was set at 4.5 kV in the positive ionization mode and the dry temperature was 180 °C. The drying gas flowed at 5.0 L/min and the nebulizer was at 0.8 bar. The MS was operated in full scan mode, and the data were collected within the range of m/z 100–2000. Samples extracted from SDB with 2 μg/mL AFB1 were used as the positive control and samples extracted from SDB and cultures of A. niger RAF106 in SDB were used as the negative control.
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