Primary Schwann cells were treated with or without 250 μM 8-(4-chlorophenylthio) adenosine-3′,5′-cyclic monophosphate (CPT-cAMP) (Sigma). After 72 h, total RNA from primary Schwann cells was extracted with TRIzol reagent (Invitrogen) according to the manufacturer’s protocol. To examine changes in miRNA expression in cAMP-treated Schwann cells, an miRNA array was carried out according to standard protocols described elsewhere [49 (link)].
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