The primary antibodies were obtained as follows. The antibodies Phospho-AMPKα (Thr172) (40H9) (2535), AMPKα (2532), AMPKα2 (2757), AMPKβ2 (4148), Phospho-Acetyl-CoA Carboxylase (Ser79) (3661), Acetyl-CoA Carboxylase (3662), E-cadherin (3195), N-cadherin (13116), PHF2 (34872), snail (3879), H3 (4499), GAPDH (2118), DYKDDDDK Tag (9A3) (8146) for immunofluorescence, Myc-Tag (9B11) (2276) were purchased from Cell Signalling Technology. The H3K9me2 (A2359), H3K9me3 (A2360), Fibronectin (A12977) for immunoblot or immunofluorescence were purchased from Abclonal Biotechnology. The PHF2 (HPA010831) antibody for immunohistochemistry were bought from Sigma-Aldrich. The H3K9me1 (PTM-614), H3K27me1 (PTM-620), H3K27me2 (PTM-621), H3K27me3 (PTM-622) for immunoblot were bought from PTM BioLab, Inc. The DYKDDDDK Tag (1E6) (018-22783) for immunoprecipitation and immunoblot was purchased from Wako. The antibody β-actin (AM1021B) was bought from Abgent.
The secondary antibodies were obtained as follows. For immunofluorescence assay, Alexa Fluor 555 labelled goat anti-mouse IgG (A21422), Alexa Fluor 555 labelled donkey anti-rabbit IgG (A31572) were purchased from Invitrogen. For immunoblot, peroxidase AffiniPure Goat Anti-Mouse IgG (H + L) (115-035-003) and Peroxidase AffiniPure Rabbit Anti-Goat IgG (H + L) (305-035-003) were purchased from Jackson ImmunoResearch Laboratories. Chemiluminescent detection was completed with chemiluminescent (ECL) western blotting reagents (GE healthcare, RPN2236). The p-PHF2 (Ser655) antibody was produced by Youke Biological Technology Co. Ltd (Shanghai, China).
The AMPK activator AICAR (HY-13417) and Metformin hydrochloride (HY-17471A) were purchased from MedChemExpress. A-769662 (CSN12424) was bought from CNSpharm. TGF-β (10804-HNAC) were purchased from SinoBiological. FGF-2 (MB2458-1) was bought from meilunbio. The siRNA was synthesised in Genepharma (Shanghai) and listed in Table S3.
Free full text: Click here