After the skin severity score had reached approximately 8–11, corresponding to moderate AD-like skin inflammation, 0.1% betamethasone ointment and 3% tetracycline ointment were applied topically to the dorsal skin and the bilateral auricle skin (50 mg/body [=50 mg/10 cm2 skin]) in each group (6 mice per group) once per day, except Sunday and any public holiday. The design of the experimental schedule is summarized in Figure 1 .
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