Protein expression and signaling analysis

Cell seeding, collection of protein and western blot methods were previously described (22 (link), 23 (link)). Membranes were probed with the following antibodies: anti-MT1-MMP (Millipore, MAB3328), anti-GAPDH (Santa Cruz Biotechnology, C65), anti-FAK-(pY397) (BD Biosciences, 611722), anti-FAK (Cell Signaling, D2R2E), anti-ITGB1 (Cell Signaling, D2E5), anti-Cleaved-PARP (Cell Signaling, D64E10), anti-SMAD3-(pS423/S425) and anti-SMAD3 (EP568Y) (Abcam).

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Marusak C., Thakur V., Li Y., Freitas J.T., Zmina P.M., Thakur V.S., Chang M., Gao M., Tan J., Xiao M., Lu Y., Mills G., Flaherty K.T., Frederick D.T., Miao B., Sullivan R.J., Moll T., Boland G., Herlyn M., Zhang G, & Bedogni B. (2020). Targeting Extracellular Matrix Remodeling restores BRAF Inhibitor Sensitivity in BRAFi Resistant Melanoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(22), 6039-6050.

Publication 2020

anti-MT1-MMP anti-GAPDH anti-FAK-(pY397) anti-FAK anti-ITGB1 anti-Cleaved-PARP

Antibodies Cell Gapdh Membranes Protein Smad3 Western blot

Corresponding Organization : University of Miami

Other organizations : University of Notre Dame, The Wistar Institute, The University of Texas MD Anderson Cancer Center, Oregon Health & Science University, Massachusetts General Hospital, Harvard University, Duke University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of MT1-MMP protein
Levels of GAPDH protein
Levels of phosphorylated FAK (pY397)
Levels of total FAK
Levels of ITGB1 protein
Levels of cleaved PARP
Levels of phosphorylated SMAD3 (pS423/S425)
Levels of total SMAD3

control variables

None explicitly mentioned

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

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