LC-MS Extraction for Vascular Plants and Bryophytes

We followed extraction procedures for LC-MS originally established by Böttcher et al. (Böttcher et al., 2009 (link)) for vascular plants and modified slightly by Peters et al. for bryophytes (Peters, Gorzolka, et al., 2018 (link)). This method was observed to give robust results regarding our targeted compound classes (Lu et al., 2021 ). Frozen plants were homogenized and extracted with 1 mL of cold 80:20 MeOH:H₂O supplemented with 5 µM Kinetin (Sigma), 5 µM Biochanin A (Sigma), and 5 µM N-(3-Indolylacetyl)-L-alanine (Sigma). For LC-MS analysis, samples were reconstituted to 10 mg fresh weight/100 µL with 80:20 MeOH:H₂O.