Proteinase K from Tritirachium album and Deoxyribonuclease I from bovine pancreas (DNAse I) were purchased from Sigma–Aldrich (France), CIP (alkaline phosphatase, calf intestinal) and E. coli exonuclease I (ExoI) were purchased from New England Biolabs France (Evry, France). SVPDE1 (snake venom phosphodiesterase 1) from Crotalus adamanteus was from Worthington (Biochemical Corporation). Human tyrosyl-DNA phosphodiesterase 1 (Tdp1) was purified as described previously (44 (link)). The purified human Nudix (nucleoside diphosphate-linked moiety X)-type motif 16 (NUDT16) protein was kindly provided by Dr Ivan Ahel (University of Oxford, U.K.).
Human poly(ADP-ribose) polymerase 1 (PARP1; EC 2.4.2.30) and bovine PARG were purchased from Trevigen (Gaithersburg, USA). The plasmid coding for murine PARP2 was kindly provided by Dr V. Schreiber (ESBS, Illkirch, France). The untagged PARP2 protein was expressed and purified from insect cells as described previously (22 (link)).