Human poly(ADP-ribose) polymerase 1 (PARP1; EC 2.4.2.30) and bovine PARG were purchased from Trevigen (Gaithersburg, USA). The plasmid coding for murine PARP2 was kindly provided by Dr V. Schreiber (ESBS, Illkirch, France). The untagged PARP2 protein was expressed and purified from insect cells as described previously (22 (link)).
Purification and Characterization of Nucleic Acid-Modifying Enzymes
Human poly(ADP-ribose) polymerase 1 (PARP1; EC 2.4.2.30) and bovine PARG were purchased from Trevigen (Gaithersburg, USA). The plasmid coding for murine PARP2 was kindly provided by Dr V. Schreiber (ESBS, Illkirch, France). The untagged PARP2 protein was expressed and purified from insect cells as described previously (22 (link)).
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Corresponding Organization :
Other organizations : Stabilité génétique et oncogenèse, Institut Gustave Roussy, Siberian Branch of the Russian Academy of Sciences, Institute of Chemical Biology and Fundamental Medicine, CEA Grenoble, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Institut Nanosciences et Cryogénie, KazMunayGas (Kazakhstan)
Variable analysis
- Proteinase K from Tritirachium album
- Deoxyribonuclease I from bovine pancreas (DNAse I)
- CIP (alkaline phosphatase, calf intestinal)
- E. coli exonuclease I (ExoI)
- SVPDE1 (snake venom phosphodiesterase 1) from Crotalus adamanteus
- Human tyrosyl-DNA phosphodiesterase 1 (Tdp1)
- Human Nudix (nucleoside diphosphate-linked moiety X)-type motif 16 (NUDT16) protein
- Human poly(ADP-ribose) polymerase 1 (PARP1; EC 2.4.2.30)
- Bovine PARG
- Murine PARP2
- Measured outcomes (not explicitly mentioned)
- Kept constant to ensure valid results (not explicitly mentioned)
- Human tyrosyl-DNA phosphodiesterase 1 (Tdp1)
- Not explicitly mentioned
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